Insertion junction: LMJ.RY0402.228840_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.228840
Chromosome:chromosome 3
Location:7971691
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g201950 (1 of 1) IPR003882//IPR008752//IPR024079 - Pistil-specific extensin-like protein // Peptidase M11, gametolysin // Metallopeptidase, catalytic domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGTGAAGCGTCTTTCTTCTTCTTCTTGGG
Internal bar code:GATCGGGCGGATACCGCGTAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:471
LEAP-Seq percent confirming:83.866
LEAP-Seq n confirming:3831
LEAP-Seq n nonconfirming:737
LEAP-Seq n unique pos:95

Suggested primers for confirmation by PCR

Suggested primer 1:TAGCTGACACAGACCGCATC
Suggested primer 2:CCTCTGCAGTCCTCCAACTC