Insertion junction: LMJ.RY0402.228867_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.228867
Chromosome:chromosome 2
Location:5482038
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g108050 (1 of 1) IPR008417 - B-cell receptor-associated protein 29/31 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCCCTTTCAATCCGCACACACACGGGCAT
Internal bar code:AGTGATCGTGTATTTGTGATTA

Confirmation - LEAP-Seq

LEAP-Seq distance:829
LEAP-Seq percent confirming:99.457
LEAP-Seq n confirming:2198
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:GAGTGTGCGTTAGGTGCTGA
Suggested primer 2:GGTGGACTCAGCTTGAGAGG