Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.228878 |
Chromosome: | chromosome_1 |
Location: | 4212783 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre01.g028000 | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CTGGCAGTAGCGTTGGTCTCAGTCCTCGTC |
Internal bar code: | AGATTCGGATCCCGGGTGTACC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1061 |
LEAP-Seq percent confirming: | 99.5876 |
LEAP-Seq n confirming: | 1932 |
LEAP-Seq n nonconfirming: | 8 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CGTGTGAGTGCCAAGAGTGT |
Suggested primer 2: | CTAGAGCTGGCTCTGGTGCT |