Insertion junction: LMJ.RY0402.228882_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.228882
Chromosome:chromosome 7
Location:3657916
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g337300 DSK2,DYRK2,DYRKP1,DYRKP,STD1 Dual-Specificity Tyrosine-Regulated Protein Kinase involved in starch degradation; (1 of 2) PTHR24058//PTHR24058:SF23 - DUAL SPECIFICITY PROTEIN KINASE // SERINE/THREONINE KINASE-RELATED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTCAATGACACCACACCGCTTCGCTGATT
Internal bar code:TGTAAGGCGCCGACGCGTGCAC

Confirmation - LEAP-Seq

LEAP-Seq distance:141
LEAP-Seq percent confirming:99.4059
LEAP-Seq n confirming:502
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CAAGCAAGGAGTCAGGAAGG
Suggested primer 2:ACCGCGAGGTATTGTACTGG