| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.228914 |
| Chromosome: | chromosome 9 |
| Location: | 6799017 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g409600 | FAP411 | Flagellar Associated Protein 411 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAACAATGCTGCAATGCCGCGTCGCAGTCG |
| Internal bar code: | CTTTACACAGCCTCAGCGGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 596 |
| LEAP-Seq percent confirming: | 99.0491 |
| LEAP-Seq n confirming: | 7083 |
| LEAP-Seq n nonconfirming: | 68 |
| LEAP-Seq n unique pos: | 100 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCACAAGAGGAGCAAACACG |
| Suggested primer 2: | CCTCCAAGCAGCAGGACTAC |