| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.228923 |
| Chromosome: | chromosome 13 |
| Location: | 1125099 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g569450 | (1 of 1) IPR000104//IPR029058 - Antifreeze protein, type I // Alpha/Beta hydrolase fold | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCACCAGCAGAAGCGCGGCAGCGCGACACC |
| Internal bar code: | CGACAGAAGGGGCGGGTCAGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 321 |
| LEAP-Seq percent confirming: | 96.1302 |
| LEAP-Seq n confirming: | 1093 |
| LEAP-Seq n nonconfirming: | 44 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGTGTGCGTTCCGATATG |
| Suggested primer 2: | CCTTCGCGTACAACTCATCC |