| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.228939 |
| Chromosome: | chromosome 16 |
| Location: | 4850655 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g686100 | (1 of 19) PTHR31251//PTHR31251:SF1 - FAMILY NOT NAMED // SQUAMOSA PROMOTER-BINDING-LIKE PROTEIN 10-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACGTTCTTTCATGCGACCGGGTGGTGTGT |
| Internal bar code: | GATCGTAAGACACAAGGTAGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 462 |
| LEAP-Seq percent confirming: | 99.6345 |
| LEAP-Seq n confirming: | 6269 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTAGCGTTGCCCGTGTAAT |
| Suggested primer 2: | GCTATAGCAGAGCTGGGGTG |