Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.228988 |
Chromosome: | chromosome_2 |
Location: | 611511 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre02.g077850 | FAP212 | Flagellar Associated Protein | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTGATCCCCGCCCAGCACGGCAGCGCATTC |
Internal bar code: | CTAGAGTCGAACGCTCAGGGGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 626 |
LEAP-Seq percent confirming: | 97.3817 |
LEAP-Seq n confirming: | 4054 |
LEAP-Seq n nonconfirming: | 109 |
LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGGACCGAAGCTTTTACCTC |
Suggested primer 2: | TACAGAGGGATGGTGAAGGC |