| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.228989 |
| Chromosome: | chromosome_5 |
| Location: | 1759416 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre05.g242500 | RSP4 | Radial spoke protein 4 | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGCTGGCGTCACGGCGGCGTCTGGCAGCGA |
| Internal bar code: | AGCTGTGGTGTTTTCGCAATGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 863 |
| LEAP-Seq percent confirming: | 99.3289 |
| LEAP-Seq n confirming: | 2516 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTGCAATAATGGCCACAC |
| Suggested primer 2: | GTTGAGCTAATACTCGCGGC |