| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.228989 |
| Chromosome: | chromosome 5 |
| Location: | 2720884 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g236802 | (1 of 71) IPR016181 - Acyl-CoA N-acyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCATGACTACGCAGCCCTGGGGGATGCA |
| Internal bar code: | AGATTGTCCCTGAGAGTGGAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 404 |
| LEAP-Seq percent confirming: | 97.9012 |
| LEAP-Seq n confirming: | 36150 |
| LEAP-Seq n nonconfirming: | 775 |
| LEAP-Seq n unique pos: | 181 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGATGGCCTGAACACGGTAG |
| Suggested primer 2: | CGGTGTGTGTGTGTGTGTGT |