Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.228999 |
Chromosome: | chromosome_12 |
Location: | 8730781 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g545900 | MAPKKK9 | antisense | 5'UTR_intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CGTGTTAACACACCGCATGCAAGCGCCACA |
Internal bar code: | TCGCTCGGAGTCAGCCGATCTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 582 |
LEAP-Seq percent confirming: | 39.9356 |
LEAP-Seq n confirming: | 744 |
LEAP-Seq n nonconfirming: | 1119 |
LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGTTCAATTCCGGTCAGCTA |
Suggested primer 2: | ACCGCACACATGAACACACT |