| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.228999 |
| Chromosome: | chromosome 12 |
| Location: | 8730781 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g545900 | MAPKKK9 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTGTTAACACACCGCATGCAAGCGCCACA |
| Internal bar code: | TCGCTCGGAGTCAGCCGATCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 582 |
| LEAP-Seq percent confirming: | 39.9356 |
| LEAP-Seq n confirming: | 744 |
| LEAP-Seq n nonconfirming: | 1119 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTTCAATTCCGGTCAGCTA |
| Suggested primer 2: | ACCGCACACATGAACACACT |