| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.229044 |
| Chromosome: | chromosome_17 |
| Location: | 3430743 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g724500 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ACAGCACATACTCCAATCCATAATTCACAG |
| Internal bar code: | CCCAATGCTACCTTTGTGTACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 569 |
| LEAP-Seq percent confirming: | 99.5138 |
| LEAP-Seq n confirming: | 614 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCAGTAGTGGGTCGGCATT |
| Suggested primer 2: | CACCGTCCTACGCTAAGCTC |