| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.229044 |
| Chromosome: | chromosome 17 |
| Location: | 5946716 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g740261 | FAP365 | (1 of 96) IPR019734 - Tetratricopeptide repeat; Flagellar Associated Protein 365 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCCCTTCCGCAGGCCGATTCTGGGACAGA |
| Internal bar code: | GGGCCGCGGGGCTGGGCCTAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 302 |
| LEAP-Seq percent confirming: | 99.8483 |
| LEAP-Seq n confirming: | 658 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCAGGAGGTACAAGCAGC |
| Suggested primer 2: | GCATGGAATGCAGGCTAAAT |