| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.229049 |
| Chromosome: | chromosome_2 |
| Location: | 6228450 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g114100 | PDE22 | 3\',5\'-cyclic-nucleotide phosphodiesterase | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCATTGGGATCCAGGGCTCCGTGCCGCAGG |
| Internal bar code: | GCATGTTTTTCGCGCTCATGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 433 |
| LEAP-Seq percent confirming: | 99.4964 |
| LEAP-Seq n confirming: | 4149 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGTGCAGTCCTGTCAAGAG |
| Suggested primer 2: | TACACAACACGGAAAGCAGC |