| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.229058 |
| Chromosome: | chromosome 1 |
| Location: | 679164 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g003541 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTCTGCGAGGCGATGCCACATTACGGCCG |
| Internal bar code: | GTTGGAGCCCTCGATGCTGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 484 |
| LEAP-Seq percent confirming: | 95.1009 |
| LEAP-Seq n confirming: | 330 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCACCCTCAAGTAGCCAG |
| Suggested primer 2: | TGCCCTTCCGGTATAGTGTC |