| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.229076 |
| Chromosome: | chromosome 4 |
| Location: | 2097389 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g219300 | (1 of 1) PF02958 - Ecdysteroid kinase (EcKinase); Putative choline kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAGAGGATTGACCGCCAGCAGGGGGGGGC |
| Internal bar code: | GTAAGATCCAGCCTAGCACGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 609 |
| LEAP-Seq percent confirming: | 99.4343 |
| LEAP-Seq n confirming: | 1582 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCCAGCATTCACTAAGCTC |
| Suggested primer 2: | AATCCCCATCCCTGAATCTC |