| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.229092 |
| Chromosome: | chromosome_6 |
| Location: | 2651432 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g270000 | sense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTACACCCTGTCTCCACACCTCCAACCACT |
| Internal bar code: | ACATGGACGCAGTCAACCTAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 549 |
| LEAP-Seq percent confirming: | 99.3305 |
| LEAP-Seq n confirming: | 1187 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCTTGCACTGCATTGTTG |
| Suggested primer 2: | GCCTGGAACTCCATCAGGTA |