| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.229093 |
| Chromosome: | chromosome 6 |
| Location: | 2499298 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g268976 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACAACTACATGTTGACGGCGGATAATTCA |
| Internal bar code: | ACTATCGGCCTTTTCCTTGACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 682 |
| LEAP-Seq percent confirming: | 98.9935 |
| LEAP-Seq n confirming: | 1967 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGTATTCAAACGTGGGGTT |
| Suggested primer 2: | CACTGCAAATCAGCCGAGTA |