| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.229098 |
| Chromosome: | chromosome 11 |
| Location: | 2905073 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g477950 | (1 of 1) IPR000104//IPR005502 - Antifreeze protein, type I // ADP-ribosylation/Crystallin J1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGCGCACATGCAGGCGGGGGGTTGTCTCT |
| Internal bar code: | CATGGCATCTTAAATTGTTGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 254 |
| LEAP-Seq percent confirming: | 99.1404 |
| LEAP-Seq n confirming: | 346 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGCAGTTGGAAGCGAGGAT |
| Suggested primer 2: | GGTGTACATCAACCACAGCG |