Insertion junction: LMJ.RY0402.229127_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.229127
Chromosome:chromosome 7
Location:493609
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g315850 (1 of 2) IPR000104//IPR000945//IPR020683 - Antifreeze protein, type I // Dopamine beta-hydroxylase-related // Ankyrin repeat-containing domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCATGCGCATGTGGCACCTGCACACCCTCG
Internal bar code:TCGTGGCGTCTCCGGGGCCCCC

Confirmation - LEAP-Seq

LEAP-Seq distance:440
LEAP-Seq percent confirming:99.4002
LEAP-Seq n confirming:1823
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:GTACCTCGGCAACCCTATCA
Suggested primer 2:CGAACAAGCACTCGCAATAA