| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.229127 |
| Chromosome: | chromosome 7 |
| Location: | 493619 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g315850 | (1 of 2) IPR000104//IPR000945//IPR020683 - Antifreeze protein, type I // Dopamine beta-hydroxylase-related // Ankyrin repeat-containing domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGGTGCCGGTGCCGGCGCTGGCCTTGCC |
| Internal bar code: | ATGAGAACATGCGCGGCAGATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 735 |
| LEAP-Seq percent confirming: | 99.3393 |
| LEAP-Seq n confirming: | 9623 |
| LEAP-Seq n nonconfirming: | 64 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACCTCGGCAACCCTATCA |
| Suggested primer 2: | CGAACAAGCACTCGCAATAA |