| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.229128 |
| Chromosome: | chromosome 2 |
| Location: | 6726762 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g118200 | (1 of 3) PF01940 - Integral membrane protein DUF92 (DUF92) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGAGTTGCTGACGGTCATTGCGGGTTCC |
| Internal bar code: | GCGGAATCTGTAACCCTGGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 517 |
| LEAP-Seq percent confirming: | 98.004 |
| LEAP-Seq n confirming: | 491 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTCTCCTCGCTTTCTCTGC |
| Suggested primer 2: | GACTAACGCAAGGGTTTCCA |