| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.229142 |
| Chromosome: | chromosome 15 |
| Location: | 1071980 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g638401 | OPR76,RAP12,CLR10 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTTAGTCCGATCATTGTGCCTACTACAGT |
| Internal bar code: | ATGAAGCGCGGCTGTGCGCTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 173 |
| LEAP-Seq percent confirming: | 95.6522 |
| LEAP-Seq n confirming: | 110 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGGGCTTGCCATTATTGT |
| Suggested primer 2: | CTTGCAGGTCCTCCAGAGTC |