| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.229148 |
| Chromosome: | chromosome 2 |
| Location: | 7556201 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g143858 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGTATGAGCCAAGCAGCAGAGCGAGTGT |
| Internal bar code: | TGGACGGTATCGCTATCGGCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 466 |
| LEAP-Seq percent confirming: | 95.7237 |
| LEAP-Seq n confirming: | 873 |
| LEAP-Seq n nonconfirming: | 39 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCACCGGACAAACCTTGAT |
| Suggested primer 2: | GACCACCTTTAGCTTGCGAC |