Insertion junction: LMJ.RY0402.229160_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.229160
Chromosome:chromosome 7
Location:5821345
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g353400 (1 of 274) IPR020683 - Ankyrin repeat-containing domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCGCCACCCAGGCGCCACGACGCCCGCCG
Internal bar code:TTGGGGCACGCTAGTGGACCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:101
LEAP-Seq percent confirming:71.0526
LEAP-Seq n confirming:27
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TGTCCGCCTACCTATCCATC
Suggested primer 2:GCCCTGTTGAGTCTTTGCTC