| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.229171 |
| Chromosome: | chromosome 10 |
| Location: | 3433536 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g444600 | (1 of 781) IPR000104 - Antifreeze protein, type I | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACATTACGTCCACACTCCACAGCTTGCCAA |
| Internal bar code: | GAGTTCTGCTAAGTTTACCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 585 |
| LEAP-Seq percent confirming: | 98.9975 |
| LEAP-Seq n confirming: | 1580 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTGAATACTGAGCGTGAA |
| Suggested primer 2: | CACGATGCATCCAGTACCAC |