Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.229175 |
Chromosome: | chromosome_3 |
Location: | 6722016 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre03.g197750 | GPX3 | Glutathione peroxidase | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CTGTCGAAGGACCCGCCACGACACCACCTG |
Internal bar code: | GCCCGGCACTTTGGGAACACCA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 753 |
LEAP-Seq percent confirming: | 97.6437 |
LEAP-Seq n confirming: | 3398 |
LEAP-Seq n nonconfirming: | 82 |
LEAP-Seq n unique pos: | 51 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CAGTCCCTGCAAGTGTCAGA |
Suggested primer 2: | AGACCACCCTTCTGGGTCTT |