Insertion junction: LMJ.RY0402.229191_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.229191
Chromosome:chromosome 2
Location:6661030
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g117600 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCGTCCCCGCCGCCCCACCACCACCCTAC
Internal bar code:CATCGTGCGGTGTAGCACGCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:339
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:315
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:GGTTAGCAACTGGCGAGAAG
Suggested primer 2:GCGTGTAGGGTTGACCTGTT