| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.229219 |
| Chromosome: | chromosome 10 |
| Location: | 5952293 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g462300 | (1 of 4) PTHR10555:SF84 - SORTING NEXIN-8 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTCACCCGCCTTGCCGCCCCTGCCGAGTC |
| Internal bar code: | CAGCGTTATCCGCACCGTGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 254 |
| LEAP-Seq percent confirming: | 68.1081 |
| LEAP-Seq n confirming: | 252 |
| LEAP-Seq n nonconfirming: | 118 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCGTTGTTTCCAAATGTCT |
| Suggested primer 2: | CTCCACCTCTACAATCGGGA |