| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.229223 |
| Chromosome: | chromosome 11 |
| Location: | 2659512 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g476600 | (1 of 5) IPR006094//IPR016166 - FAD linked oxidase, N-terminal // FAD-binding, type 2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTGCTTGCTCGTAATATTTGGGTGGCAG |
| Internal bar code: | ACTGTCCCACGACAGGCAGTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 594 |
| LEAP-Seq percent confirming: | 98.8805 |
| LEAP-Seq n confirming: | 4858 |
| LEAP-Seq n nonconfirming: | 55 |
| LEAP-Seq n unique pos: | 68 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGGACTACAAGACCCGTT |
| Suggested primer 2: | GCGCAACAGAAAGAATGACA |