| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.229246 |
| Chromosome: | chromosome 16 |
| Location: | 4730377 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g686850 | PTA4 | (1 of 10) IPR005828//IPR020846 - Major facilitator, sugar transporter-like // Major facilitator superfamily domain; Proton/phosphate symporter | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGTGCGGCCGTCCACAAGGCCAAACACA |
| Internal bar code: | CCGTGTCGGATGGGTTTTGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 800 |
| LEAP-Seq percent confirming: | 98.7033 |
| LEAP-Seq n confirming: | 1294 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGCTACTACTTTGCCGCTT |
| Suggested primer 2: | TGAACCCTACTTCTGGACCG |