| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.229249 |
| Chromosome: | chromosome 1 |
| Location: | 2389965 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g013100 | PRL10 | (1 of 14) PTHR10334 - CYSTEINE-RICH SECRETORY PROTEIN-RELATED; Predicted extracellular protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATAAACAACTCGGACCCGGACCTGTCATG |
| Internal bar code: | CCAAAAGGCAATTATTCGACGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 472 |
| LEAP-Seq percent confirming: | 97.5387 |
| LEAP-Seq n confirming: | 8758 |
| LEAP-Seq n nonconfirming: | 221 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACACGCACACGCTCTTAC |
| Suggested primer 2: | CCAGGAATCAGAGGGAATCA |