| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.229256 |
| Chromosome: | chromosome 12 |
| Location: | 7453028 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g556350 | CFAP300 | (1 of 1) PTHR31339//PTHR31339:SF10 - FAMILY NOT NAMED // PECTIN LYASE-LIKE SUPERFAMILY PROTEIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACACAGAAACGCGCACGCCACCAGGTTC |
| Internal bar code: | CCGGCACACAACCGTTTCGATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 311 |
| LEAP-Seq percent confirming: | 99.7242 |
| LEAP-Seq n confirming: | 7231 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGAGGCAGGATACGTAGTG |
| Suggested primer 2: | TAGCTTGAGACGTGAGGGGT |