| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.229266 |
| Chromosome: | chromosome 1 |
| Location: | 2454620 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g013800 | SXD1,TCY1 | (1 of 1) PF14392 - Zinc knuckle (zf-CCHC_4); Zinc finger containing protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTGGGAGCCTAGACCGCCTGGTGCCAGG |
| Internal bar code: | CTTCTAGGCTAGAGTGTGCTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 386 |
| LEAP-Seq percent confirming: | 98.9917 |
| LEAP-Seq n confirming: | 1669 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTTGCACCATTCCACAGT |
| Suggested primer 2: | TGAGATGACCAAGCTGTTGC |