| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.229271 |
| Chromosome: | chromosome 3 |
| Location: | 2059278 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g156550 | (1 of 1) PTHR22829//PTHR34826 - DEP DOMAIN PROTEIN // FAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAGCACGCCGGGTGAGCAGCAACAGCAAC |
| Internal bar code: | CCTCCCTTGTCTTGCGCGGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 524 |
| LEAP-Seq percent confirming: | 77.1746 |
| LEAP-Seq n confirming: | 111870 |
| LEAP-Seq n nonconfirming: | 33087 |
| LEAP-Seq n unique pos: | 291 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTGTGTGCGTTTGTGTGT |
| Suggested primer 2: | GGAGGGACAACAGGACAAAA |