Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.229298 |
Chromosome: | chromosome_2 |
Location: | 3530023 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre02.g095126 | DPE2 | alpha-1,4-glucanotransferase | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | AAGATTGCGGCACGCCATGAAAGATCGCGC |
Internal bar code: | TGGCGTTGTCACCGGGCGCTTT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 231 |
LEAP-Seq percent confirming: | 99.373 |
LEAP-Seq n confirming: | 1585 |
LEAP-Seq n nonconfirming: | 10 |
LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGTCTCTCCTACCCGACACC |
Suggested primer 2: | ACACACACACACGCACACAC |