| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.229317 |
| Chromosome: | chromosome 1 |
| Location: | 6338840 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g045902 | HCF101 | [4Fe-4S]-cluster scaffold protein; (1 of 1) PTHR23264//PTHR23264:SF29 - NUCLEOTIDE-BINDING PROTEIN NBP35 YEAST -RELATED // PROTEIN HIGH CHLOROPHYLL FLUORESCENCE 101 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCAAGCCGCGGCCTGCCGCGCCCCTTGGT |
| Internal bar code: | GAGAGGACGTACACTGGTGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 592 |
| LEAP-Seq percent confirming: | 99.4179 |
| LEAP-Seq n confirming: | 2391 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 58 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTTGGCTTGGGTGACTTC |
| Suggested primer 2: | TAATGCGGAGGAAATTCGAC |