| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.229318 |
| Chromosome: | chromosome 12 |
| Location: | 3875634 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g515650 | EIF3K | Eukaryotic translation initiation factor 3, subunit K; (1 of 1) K15028 - translation initiation factor 3 subunit K (EIF3K) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACGCTGCGCCACAGGCGCTGCAGCTGGTC |
| Internal bar code: | GGGGGCTTACGACGTTATCTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 336 |
| LEAP-Seq percent confirming: | 99.3795 |
| LEAP-Seq n confirming: | 961 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCGTATGAACGCTGATGTG |
| Suggested primer 2: | CTACAAGCTCACGCCATTCA |