| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.229325 |
| Chromosome: | chromosome 1 |
| Location: | 3638925 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g023500 | FAL2 | Similar to Flagellar Associated Protein FAP234 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCACACCGCCCTCCACATTGCCCACCGCCC |
| Internal bar code: | AAGTATGCCAGTTGTGTGTGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 362 |
| LEAP-Seq percent confirming: | 85.6832 |
| LEAP-Seq n confirming: | 2364 |
| LEAP-Seq n nonconfirming: | 395 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCTTGGCAGCAGTAACAG |
| Suggested primer 2: | TGCACATCTTTTCGTTGCTC |