| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.229335 |
| Chromosome: | chromosome 16 |
| Location: | 4279215 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g668150 | (1 of 31) IPR003590 - Leucine-rich repeat, ribonuclease inhibitor subtype | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGACGACAGGCAGGGCATGGGATGGCAAG |
| Internal bar code: | GCTTGTGTGTCAGTGTGCACGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 735 |
| LEAP-Seq percent confirming: | 98.9422 |
| LEAP-Seq n confirming: | 1216 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGAACCTTTGCAATCTGGC |
| Suggested primer 2: | AGCTCCTCCTGCACACAAGT |