| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.229350 |
| Chromosome: | chromosome 12 |
| Location: | 6894520 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g560750 | (1 of 10) PF02536 - mTERF (mTERF) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGGGCGGCTCACAGCAGCAGGTATGCGA |
| Internal bar code: | TAATGCGTTGTGGGGGTCGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1005 |
| LEAP-Seq percent confirming: | 98.8186 |
| LEAP-Seq n confirming: | 20158 |
| LEAP-Seq n nonconfirming: | 241 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACATCATCAACCCTGCAC |
| Suggested primer 2: | CCCCTGATCCTCCCTCTAAC |