| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.229372 |
| Chromosome: | chromosome 13 |
| Location: | 3045999 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g584450 | (1 of 6) PTHR27000:SF160 - LEUCINE-RICH REPEAT-CONTAINING PROTEIN DDB_G0281931-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCATGCTCGTGTGGGCTTCAATGCTGTGG |
| Internal bar code: | GCGTTCGTGTCATACGTAAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 496 |
| LEAP-Seq percent confirming: | 98.6805 |
| LEAP-Seq n confirming: | 1047 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACAGCAGCATCACACAGG |
| Suggested primer 2: | AAGTGACGCCAATCCACTTG |