| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.229409 |
| Chromosome: | chromosome 14 |
| Location: | 4143719 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g634236 | 5'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCCCGACCACACACCCCCGACCCCAAAA |
| Internal bar code: | GCAGCATGCACCAAGGTGAGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 141 |
| LEAP-Seq percent confirming: | 70.5882 |
| LEAP-Seq n confirming: | 72 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAATGGGAACTCGTTTGACG |
| Suggested primer 2: | GCTATCTTAAGGCAGTGGCG |