| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.229409 |
| Chromosome: | chromosome_14 |
| Location: | 4143737 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre14.g634236 | sense | 5'UTR|outside_mRNA |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTTGGTCAAACGTAGCTTGGTCAAAGTTTG |
| Internal bar code: | CGATACACGGCACGTTGGAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 706 |
| LEAP-Seq percent confirming: | 86.0279 |
| LEAP-Seq n confirming: | 431 |
| LEAP-Seq n nonconfirming: | 70 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAATGGGAACTCGTTTGACG |
| Suggested primer 2: | GCTATCTTAAGGCAGTGGCG |