Insertion junction: LMJ.RY0402.229416_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.229416
Chromosome:chromosome 1
Location:108820
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre01.g000750 ZFR2 zinc finger protein; (1 of 93) 6.3.2.19 - Transferred entry: 2.3.2.23, 2.3.2.27 and 6.2.1.45 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATTGACTCCGCCTTTCATGTCTGGCTCCCC
Internal bar code:GTTGTCTGCGTAGTTGTGTATT

Confirmation - LEAP-Seq

LEAP-Seq distance:21
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:449
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GTAACGGTGTTGTGGTGGTG
Suggested primer 2:GACTAATGGCCATACGGTGG