| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.229422 |
| Chromosome: | chromosome 12 |
| Location: | 4473734 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g521650 | (1 of 3) PTHR22753:SF1 - TRANSMEMBRANE PROTEIN 68 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGAGGCTGCAGGTGCGGCGAGGAGGGCGG |
| Internal bar code: | CCCCGGCTGTGCAACTCCAATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 382 |
| LEAP-Seq percent confirming: | 79.9306 |
| LEAP-Seq n confirming: | 3457 |
| LEAP-Seq n nonconfirming: | 868 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTACATGAACGGCTACCCC |
| Suggested primer 2: | CTCTTTCTCCACACATCGCA |