Insertion junction: LMJ.RY0402.229427_3

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.229427
Chromosome:chromosome 14
Location:1073347
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre14.g614850 FAP33,ANK17 Flagellar Associated Protein 33; (1 of 78) IPR002110//IPR020683 - Ankyrin repeat // Ankyrin repeat-containing domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCCGCGAAACTGCGAAACCTTGTACACTC
Internal bar code:AACCTTGGACGGCAAGCGATTC

Confirmation - LEAP-Seq

LEAP-Seq distance:151
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:73
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CCGCACACATTCATTCATTC
Suggested primer 2:GGTAGGGTGCGGTGAAGTTA