| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.229430 |
| Chromosome: | chromosome 6 |
| Location: | 5243912 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g282300 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCAGATGAGGAGGGCGTACGACCGAAGAA |
| Internal bar code: | GGGGACGAGTGTTCTACGCGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 585 |
| LEAP-Seq percent confirming: | 95.509 |
| LEAP-Seq n confirming: | 638 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGACTGAGACTCGCCCTAAC |
| Suggested primer 2: | CTTTCGCTGCACCAGACATA |