| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.229440 |
| Chromosome: | chromosome 10 |
| Location: | 4251797 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g450400 | NUOE,NUO5 | (1 of 1) K03943 - NADH dehydrogenase (ubiquinone) flavoprotein 2 (NDUFV2); NADH:ubiquinone oxidoreductase 24 kDa subunit | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACATACGTAAAAGCTCCTCACACATACCC |
| Internal bar code: | TCCGCGGCCAGGGTGGCGGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 620 |
| LEAP-Seq percent confirming: | 97.769 |
| LEAP-Seq n confirming: | 745 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCTCGAACTGGTCTGGCTT |
| Suggested primer 2: | TATAGCCAGGATTTGTCGGC |