| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.229456 |
| Chromosome: | chromosome 9 |
| Location: | 5701755 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g401552 | (1 of 2) IPR000104//IPR002893//IPR003072 - Antifreeze protein, type I // Zinc finger, MYND-type // Orphan nuclear receptor, NOR1 type | CDS|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGTAACAACGTACGACAGCAGTAGCGGCA |
| Internal bar code: | GACTGCGAAGCAACCCGGCAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 412 |
| LEAP-Seq percent confirming: | 99.096 |
| LEAP-Seq n confirming: | 877 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAAGCATCCTAAGCCAAAG |
| Suggested primer 2: | CTGTCGATCCCGGACATAGT |